Miniaturized modules for light sheet microscopy with low chromatic aberration.
نویسندگان
چکیده
Two miniaturized fibre-coupled modules for light sheet-based microscopy are described and compared with respect to image quality, chromatic aberration and beam alignment. Whereas in one module the light sheet is created by an achromatic cylindrical lens, reflection by a spherical mirror and concomitant astigmatic distortion are used to create the light sheet in the second module. Test experiments with fluorescent dyes in solution and multicellular tumour spheroids are reported, and some details on construction are given for both systems. Both modules are optimized for imaging individual cell layers of 3D biological samples and can be adapted to fit commercial microscopes.
منابع مشابه
Miniaturization and defocus correction for objective-coupled planar illumination microscopy.
Recently, a light sheet-based technique called objective-coupled planar illumination (OCPI) microscopy [Holekamp et al., Neuron 57, 661 (2008)] was shown to permit low-phototoxicity, high-speed, three-dimensional fluorescence imaging of extended tissue samples. Here, we introduce two major improvements in OCPI microscopy. First, we miniaturize the objective coupler by using a uniaxial gradient-...
متن کاملReaching a new resolution standard with electron microscopy
The invention of spherical aberration correctors has been the most significant contribution to the field of transmission electron microscopy since the field emission gun. Chromatic and spherical aberration, well known from optics, also play a role in electron microscopy. Lens aberrations are not unique to the magnetic lenses used in electron microscopes, but rather a fundamental problem of opti...
متن کاملNew possibilities with aberration-corrected electron microscopy.
Unlike light microscopy, where resolution is diffraction limited, the achievable resolution of electron microscopes is limited by lens aberrations so severe that the practical resolution is orders of magnitude worse than the diffraction limit. About 10 years ago, the first practical electron lens spherical aberration correctors were developed, and in the past decade, their performance, versatil...
متن کاملChromatic confocal microscopy for multi-depth imaging of epithelial tissue
We present a novel chromatic confocal microscope capable of volumetric reflectance imaging of microstructure in non-transparent tissue. Our design takes advantage of the chromatic aberration of aspheric lenses that are otherwise well corrected. Strong chromatic aberration, generated by multiple aspheres, longitudinally disperses supercontinuum light onto the sample. The backscattered light dete...
متن کاملTheoretical estimates of spherical and chromatic aberration in photoemission electron microscopy.
We present theoretical estimates of the mean coefficients of spherical and chromatic aberration for low energy photoemission electron microscopy (PEEM). Using simple analytic models, we find that the aberration coefficients depend primarily on the difference between the photon energy and the photoemission threshold, as expected. However, the shape of the photoelectron spectral distribution impa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of microscopy
دوره 264 3 شماره
صفحات -
تاریخ انتشار 2016